IsoSTED microscopy is a clever combination of STED and 4Pi microscopy and reduces the (cigar-shaped) confocal volume to a sphere of only 30 nm diameter. This corresponds to a reduction of the focal volume by three orders of magnitude [Schmidt et al., 2009].
The coherent use of two opposing objective lenses (4Pi configuration) renders an almost isotropic resolution in all three spatial dimensions.
This “3D-nanosope” was the first light microscope to unravel the interior of mitochondria (the cellular power plants) in intact cells [Schmidt et al., 2008].
It was therefore possible to image the distribution of two proteins, one forming clusters on the mitochondrial membrane, the other being distributed in the interior of the organelle, with unprecedented resolution.
- R. Schmidt, C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, S. W. Hell (2008):
"Spherical nanosized focal spot unravels the interior of cells"
Nature Meth. 5 (6), 539 - 544
- R. Schmidt, C. A. Wurm, A. Punge, A. Egner, S. Jakobs, S. W. Hell (2009):
"Mitochondrial Cristae Revealed with Focused Light"
Nano Lett. 9 (6), 2508-2510
- S. W. Hell, R. Schmidt, A. Egner (2009):
"Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses"
Nature Photonics 3, 381 - 387
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